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goat anti mouse 412 lyve1 antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat anti mouse 412 lyve1 antibody
    Goat Anti Mouse 412 Lyve1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+lyve+1/Mouse+LYVE-1+Antibody/pm41885968-208-25-30
    Average 94 stars, based on 263 article reviews
    goat anti mouse 412 lyve1 antibody - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice
    Article Snippet: The following antibodies were used: goat anti-LYVE-1 (polyclonal, R&D, AF2089), rat anti-PECAM-1 (clone 5D2.6 and IG5.1, provided by D. Vestweber, Max Planck Institute for Molecular Biomedicine Munster), rabbit anti-LYVE-1 (polyclonal, Reliatech), goat anti-LDLR (polyclonal, R&D, AF2255), rabbit anti-GS (polyclonal, Sigma-Aldrich, G2781), rabbit anti-CK19 (polyclonal, proteintech, 14965-1-AP), mouse anti-smooth muscle actin (SMA)-Cy3 (clone 1A4, Sigma), rat anti-Lyve-AlexaFluor594 (clone 223322, R&D), secondary antibodies coupled to AlexaFluor488, 568 and 647 (Invitrogen).

    Article Title: A tunnel microtract organ for T cell progenitor homing is formed by neural crest morphogenesis via Sox10-Cdc42 axis
    Article Snippet: The primary antibodies were utilized: goat anti-GFP (1:400, Abcam, ab6658), rabbit anti-DsRed (1:400, Takara, 632496), Mouse anti-II-II6B3 (1:400, DSHB, AB_528165); Mouse anti-S58 (1:400, DSHB, AB_528377); Mouse anti-F310 (1:400, DSHB, AB_531863); rabbit anti-SOX10 (1:400, Abcam, ab155279), rabbit anti-FOXN1 (1:400, UABIO, ER1908-98), goat anti-LYVE-1 (1:400, R&D Systems, AF2125), mouse anti-CD31 (1:400, HUABIO, M1511-8), rat anti-CD34 (1:400, BD Biosciences, 553731); mouse anti-CD45 (1:400, proteintech, 60287-1-Ig); rabbit anti-CD45 (1:400, Cell Signaling Technology, 70257); rabbit anti-SOX10 (1:400, Abcam, AB_2650603); rabbit anti-CDC42 (1:400, Huabio, AB_3070234); rabbit anti-SOX9 (1:400, Abcam, AB_2728660); rabbit anti-IKZF1 (1:400, Huabio, AB_2124704); Lcp1 antibody (1:400, GeneTex, AB_11167454); rabbit anti-TBX5 (1:400, Novus, AB_11018767); rabbit anti-HOXD11 (1:400, Novus, AB_3343027).

    Article Title: An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth
    Article Snippet: Primary antibodies were as follows: rat anti-CD31 (BD Biosciences, San Jose, CA, USA), rabbit anti-LYVE-1 (Angiobio, Del Mar, CA, USA), goat anti-LYVE-1 (R&D Systems), rat anti-CD68 (Abcam, Cambridge, MA, USA), goat anti-podoplanin (R&D Systems), rabbit anti-cytokeratin 15 (Abcam), rat anti-Foxp3 (eBioscience, San Diego, CA, USA), and goat anti-Prox1 (R&D systems).

    Incubation:

    Article Title: Impaired meningeal lymphatic vessel development worsens stroke outcome
    Article Snippet: Meninges were washed with PBS and then blocked overnight with PBSþ 0.3% TX-100þ 20% Aquablock (East Coast Bio, North Berwick, ME, USA). .. Primary antibody was then added and the meninges were incubated overnight at 4 C. The following primary antibodies were used: rat anti-CD31 (1:1000; eBiosciences, San Diego, CA, USA), goat anti-Lyve-1 (1:1000; R&D Systems, Minneapolis, MN, USA), hamster anti-podoplanin (1:1000; Abcam, San Francisco, CA, USA), and rabbit anti-Prox1 (1:500; AngioBioCo, Del Mar, CA, USA). ..

    Article Title: Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease.
    Article Snippet: At the level of the hippocampus (bregma −1.58 mm to −2.46 mm), serial coronal sections (30 μm) were obtained from each brain with a freezing microtome (Leica Microsystems, Inc., Exton, PA, USA), and stored in PBS at 4°C prior to immunostaining. .. Dura and deep cervical lymph node sections were washed twice with PBS for 5 minutes each and blocked in PBS containing 1% bovine serum albumin and 0.25% Triton X-100 (Sigma-Aldrich, St Louis, MO, USA) for 1 hour at 37°C and subsequently incubated overnight at 4°C with appropriate dilutions of primary antibodies: goat anti-Lyve-1 (1:600; R&D Systems) and rabbit anti-Prox1 (1:500; Abcam). ..

    Article Title: Regulation of lymphangiogenesis in the diaphragm by macrophages and VEGFR-3 signaling
    Article Snippet: .. The following antibodies were incubated overnight: Rabbit anti-LYVE-1 (AngioBio), goat anti-LYVE-1 (R&D Systems), rat anti-CD31 (BD Biosciences Pharmingen), goat anti-CD206 (R&D Systems), mouse anti-smooth muscle actin (Sigma-Aldrich), rat anti-CD68 (Abcam), goat anti-Prox-1 (R&D Systems), rabbit anti-Prox-1 (kind gift of Dr. Kari Alitalo), rat anti-F4/80 (Abcam) and rat anti-CSF-1R (AFS98 hybridoma antibody). .. After an intense washing step, tissues were incubated for 2 h at room temperature with Alexa Fluor 488, 594 or 647 nm-conjugated secondary antibodies (Invitrogen) and mounted in Vectashield (Vector) for confocal imaging.

    Labeling:

    Article Title: Single-nuclear transcriptomics of lymphedema-associated adipose reveals a pro-lymphangiogenic stromal cell population
    Article Snippet: .. Primary antibody and dilutions used for labeling were: Alexa Fluor 488 rabbit anti-PLIN1 (1:100, Cell Signaling, 29138S), goat anti-LYVE 1 (1:100, R&D Systems, AF2089), rabbit anti-CD31 (1:200, Proteintech, 11265-1-AP), and FITC GRIA1 (1:50, Thermo Scientific, MA45628). ..

    Immunohistochemistry:

    Article Title: Meningeal macrophages regulate fibroblasts to influence meningeal lymphatic function following traumatic brain injury
    Article Snippet: Finally, samples were mounted with DAPI-containing antifade mounting medium (ab104139, Abcam) and imaged with a Leica DMi8 confocal microscope. .. The primary antibodies used for immunohistochemistry included: Rat Anti-CD31 (1:100, sc-18916, Santa Cruz), Rabbit Anti-VEGF-C (1:200, ab9546, Abcam), Rat Anti-PDGFRα (1:200, 14-1401-82, Thermo Fisher Scientific), Goat anti-CD206 (1:200, AF2535, R&D Systems), Rat anti-LYVE-1 (1:100, 14-0443-82, Thermo Fisher Scientific), Goat anti-LYVE-1 (1:200, AF2125, R&D Systems), Rat anti-CD163 (1:200, 14-1631-82, Thermo Fisher Scientific), Rabbit Anti-CCR2 (1:200, ab273050, Abcam), Rabbit Anti-CD68 (1:200, PA578996 , Thermo Fisher Scientific), Goat anti-IBA-1 (1:200, ab5076, Abcam), Rabbit anti-IBA-1 (1:500, 019-19741, Wako), Rat anti-MBP (1:200, ab7349, Abcam), Mouse anti-SMI32 (1:200, 801701, BioLegend), Rabbit Anti-GFAP (1:200, 80788, CST), and Goat anti-EphB4 (1:200, AF446, R&D Systems). ..



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    R&D Systems goat anti mouse 412 lyve1 antibody
    Goat Anti Mouse 412 Lyve1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems goat anti mouse lymphatic vessel hyaluronic acid receptor 1
    LVD quantification using IHC on the hearts of transgenic mice with perturbed GH signaling. (A) Hematoxylin and eosin staining of mouse heart sections from each genotype. (B) Representative images from IHC staining using 2 primary antibodies against <t>LYVE1</t> and CD31. Fluorescent detection using a red Cy5 or green Alexa488 fluorophore bound to a secondary antibody was used to visualize LYVE1 and CD31, respectively. (C) Fluorescent IHC combined with brightfield imaging using the previously mentioned antibodies toward specific protein targets (LYVE1 and CD31) and using isotype control antibodies. (D, E) The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 3 mice per group (n = 3). The mean LVD ± SEM is shown in each bar graph. Group comparisons were performed using an unpaired t -test, with P -values displayed above the graph. Abbreviations: IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1.
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    R&D Systems goat
    LVD quantification using IHC on the hearts of transgenic mice with perturbed GH signaling. (A) Hematoxylin and eosin staining of mouse heart sections from each genotype. (B) Representative images from IHC staining using 2 primary antibodies against <t>LYVE1</t> and CD31. Fluorescent detection using a red Cy5 or green Alexa488 fluorophore bound to a secondary antibody was used to visualize LYVE1 and CD31, respectively. (C) Fluorescent IHC combined with brightfield imaging using the previously mentioned antibodies toward specific protein targets (LYVE1 and CD31) and using isotype control antibodies. (D, E) The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 3 mice per group (n = 3). The mean LVD ± SEM is shown in each bar graph. Group comparisons were performed using an unpaired t -test, with P -values displayed above the graph. Abbreviations: IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1.
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    R&D Systems goat polyclonal anti mlyve1
    LVD quantification using IHC on the hearts of transgenic mice with perturbed GH signaling. (A) Hematoxylin and eosin staining of mouse heart sections from each genotype. (B) Representative images from IHC staining using 2 primary antibodies against <t>LYVE1</t> and CD31. Fluorescent detection using a red Cy5 or green Alexa488 fluorophore bound to a secondary antibody was used to visualize LYVE1 and CD31, respectively. (C) Fluorescent IHC combined with brightfield imaging using the previously mentioned antibodies toward specific protein targets (LYVE1 and CD31) and using isotype control antibodies. (D, E) The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 3 mice per group (n = 3). The mean LVD ± SEM is shown in each bar graph. Group comparisons were performed using an unpaired t -test, with P -values displayed above the graph. Abbreviations: IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1.
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    Image Search Results


    LVD quantification using IHC on the hearts of transgenic mice with perturbed GH signaling. (A) Hematoxylin and eosin staining of mouse heart sections from each genotype. (B) Representative images from IHC staining using 2 primary antibodies against LYVE1 and CD31. Fluorescent detection using a red Cy5 or green Alexa488 fluorophore bound to a secondary antibody was used to visualize LYVE1 and CD31, respectively. (C) Fluorescent IHC combined with brightfield imaging using the previously mentioned antibodies toward specific protein targets (LYVE1 and CD31) and using isotype control antibodies. (D, E) The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 3 mice per group (n = 3). The mean LVD ± SEM is shown in each bar graph. Group comparisons were performed using an unpaired t -test, with P -values displayed above the graph. Abbreviations: IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1.

    Journal: Endocrinology

    Article Title: GH Alters Lymphatic Vessels in Female Mice and STAT5 Phosphorylation in Human Lymphatic Endothelial Cells

    doi: 10.1210/endocr/bqaf194

    Figure Lengend Snippet: LVD quantification using IHC on the hearts of transgenic mice with perturbed GH signaling. (A) Hematoxylin and eosin staining of mouse heart sections from each genotype. (B) Representative images from IHC staining using 2 primary antibodies against LYVE1 and CD31. Fluorescent detection using a red Cy5 or green Alexa488 fluorophore bound to a secondary antibody was used to visualize LYVE1 and CD31, respectively. (C) Fluorescent IHC combined with brightfield imaging using the previously mentioned antibodies toward specific protein targets (LYVE1 and CD31) and using isotype control antibodies. (D, E) The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 3 mice per group (n = 3). The mean LVD ± SEM is shown in each bar graph. Group comparisons were performed using an unpaired t -test, with P -values displayed above the graph. Abbreviations: IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1.

    Article Snippet: The primary antibodies were prepared in TBS-T with 1% BSA at the following concentrations: 4 ug/mL of goat anti-mouse lymphatic vessel-hyaluronic acid receptor 1 (LYVE1; R&D Systems Cat# AF2125, RRID: AB_2297188 ), 3 ug/mL of rabbit anti-mouse CD31 (Proteintech Cat# 28083-1-AP, RRID: AB_2881055 ), or 4 ug/mL goat isotype control antibody (R&D Systems Cat# AB-108-C, RRID: AB_354267 ) or 3 ug/mL rabbit isotype control antibodies (R&D Systems Cat# AB-105-C, RRID: AB_354266 ).

    Techniques: Transgenic Assay, Staining, Immunohistochemistry, Imaging, Control

    Skin histology of the uncut and cut sides 1 month after surgery in transgenic mice with altered GH signaling. Representative H&E and fluorescent IHC images of cut (A) and uncut (B) skin from the bGH, WT littermate from the GHA cohort, and GHA mice. The H&E image is of the full skin thickness at 10×, while the IHC image is of the dermis at 20×. The IHC staining used 1 primary antibody against LYVE1 and 1 secondary conjugated to the Cy5 fluorophore along with DAPI staining of the nuclei. The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 2 to 3 mice per group (n = 2-3). To assess statistical differences between the cut and uncut sides and between WT and transgenic animals, a mixed-effects 2-way ANOVA was used. No statistical difference in LVD was observed between WT and transgenic animals in the dermis or hypodermis or between the cut and uncut sides in the bGH (C) and GHA (E) groups; therefore, P -values from post hoc analysis were not calculated or displayed. Bar graphs represent mean ± SEM. (D) Brightfield imaging was combined with fluorescent imaging of LYVE1 staining or isotype control antibody staining to assess for antibody specificity. Abbreviations: bGH, bovine GH; DAPI, 4',6-diamidino-2-phenylindole; GHA, GH antagonist; H&E, hematoxylin and eosin; IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1; WT, wild-type.

    Journal: Endocrinology

    Article Title: GH Alters Lymphatic Vessels in Female Mice and STAT5 Phosphorylation in Human Lymphatic Endothelial Cells

    doi: 10.1210/endocr/bqaf194

    Figure Lengend Snippet: Skin histology of the uncut and cut sides 1 month after surgery in transgenic mice with altered GH signaling. Representative H&E and fluorescent IHC images of cut (A) and uncut (B) skin from the bGH, WT littermate from the GHA cohort, and GHA mice. The H&E image is of the full skin thickness at 10×, while the IHC image is of the dermis at 20×. The IHC staining used 1 primary antibody against LYVE1 and 1 secondary conjugated to the Cy5 fluorophore along with DAPI staining of the nuclei. The LYVE1-positive area and total area were measured using a pixel classifier in QuPath across 3 to 5 sections per mouse from 2 to 3 mice per group (n = 2-3). To assess statistical differences between the cut and uncut sides and between WT and transgenic animals, a mixed-effects 2-way ANOVA was used. No statistical difference in LVD was observed between WT and transgenic animals in the dermis or hypodermis or between the cut and uncut sides in the bGH (C) and GHA (E) groups; therefore, P -values from post hoc analysis were not calculated or displayed. Bar graphs represent mean ± SEM. (D) Brightfield imaging was combined with fluorescent imaging of LYVE1 staining or isotype control antibody staining to assess for antibody specificity. Abbreviations: bGH, bovine GH; DAPI, 4',6-diamidino-2-phenylindole; GHA, GH antagonist; H&E, hematoxylin and eosin; IHC, immunohistochemistry; LVD, lymphatic vascular density; LYVE1, lymphatic vessel-hyaluronic acid receptor 1; WT, wild-type.

    Article Snippet: The primary antibodies were prepared in TBS-T with 1% BSA at the following concentrations: 4 ug/mL of goat anti-mouse lymphatic vessel-hyaluronic acid receptor 1 (LYVE1; R&D Systems Cat# AF2125, RRID: AB_2297188 ), 3 ug/mL of rabbit anti-mouse CD31 (Proteintech Cat# 28083-1-AP, RRID: AB_2881055 ), or 4 ug/mL goat isotype control antibody (R&D Systems Cat# AB-108-C, RRID: AB_354267 ) or 3 ug/mL rabbit isotype control antibodies (R&D Systems Cat# AB-105-C, RRID: AB_354266 ).

    Techniques: Transgenic Assay, Immunohistochemistry, Staining, Imaging, Control